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All samples were then supplemented with 1 mM PMSF to inactivate proteinase K, volumes normalized with 1X TD, and prepared for SDS-PAGE

relapse risk during this window is highest without structured treatment support Medication-Assisted Treatment for Hydrocodone Use Disorder Medication-assisted treatment (MAT) combines FDA-approved pharmacotherapy with behavioral therapy to reduce withdrawal severity, suppress cravings, and prevent fatal relapse during the high-vulnerability post-acute period

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Instead, it is plausible that the combined increase of skeletal muscle oxidative capacity and C/F ratio led to improved oxygen extraction compared to placebo, as discussed above